Composite

Part:BBa_K4889004:Design

Designed by: zhaoxia zhang   Group: iGEM23_Shanghai-MedX   (2023-09-06)


Plac-GST-MST2 1-308


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1685
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 939
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1616
    Illegal SapI.rc site found at 169


Design Notes

The part include T7 promoter, lac operator, RBS, GST tag, MST2 (1-308), and T7 terminator. MST2 was digested with restriction endonucleases (NcoI and XhoI) and ligated into the plasmid containing the other components using T4 DNA ligase.


Source

The part is Composite pasts.

References